IL-1 receptor antagonist (IL-1Ra) prevents murine bronchopulmonary dysplasia (BPD) induced by perinatal inflammation and hyperoxia. — ASN Events

IL-1 receptor antagonist (IL-1Ra) prevents murine bronchopulmonary dysplasia (BPD) induced by perinatal inflammation and hyperoxia. (#309)

Marcel Nold 1 2 , Niamh Mangan 3 , Ina Rudloff 1 2 , Steven Cho 1 , Nikeh Shariatrian 1 , Thilini Samarasinghe 1 , Elizabeth Skuza 1 , John Pedersson 4 , Alex Veldman 5 , Philip Berger 1 , Claudia Nold-Petry 1 2
  1. The Ritchie Centre, MIMR-PHI Institute of Medical Research , Melbourne, VIC, Australia
  2. Department of Pediatrics, Monash University, Melbourne, VIC, Australia
  3. Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Melbourne, VIC, Australia
  4. TissuPath, Mount Waverly, VIC, Australia
  5. Monash Institute of Medical Research, Clayton, VIC, Australia

BPD is a common lung disease of premature infants, with devastating short- and long-term consequences. The pathogenesis of BPD is multi-factorial, but all triggers cause pulmonary inflammation. No therapy exists; thus, we investigated whether the anti-inflammatory IL-1Ra prevents murine BPD.

We precipitated BPD by perinatal inflammation (LPS injection to pregnant dams) and rearing pups in hyperoxia (65% or 85% O2). Pups were treated daily with IL-1Ra or vehicle.

Vehicle-injected animals in both levels of hyperoxia developed a severe BPD-like lung disease (alveolar number and gas exchange area decreased by 60%, alveolar size increased 4-fold). IL-1Ra prevented this structural disintegration at 65%, but not 85% O2. Hyperoxia depleted pulmonary immune cells by 67%; however, extant macrophages and dendritic cells were hyper-activated, with CD11b and GR1 highly expressed. IL-1Ra partially rescued the immune cell population in hyperoxia (doubling viable cells), reduced the percentage that were activated by 63%, and abolished the unexpected persistence of IL-1alpha and IL-1beta on d28 in hyperoxia/vehicle-treated lungs. On d3, perinatal inflammation and hyperoxia each triggered a distinct pulmonary immune response, with some pro-inflammatory mediators increasing 20-fold and some amenable to partial or complete reversal with IL-1Ra.

In summary, our analysis reveals a pivotal role for IL-1 in murine BPD and an involvement for MIP-1 and TREM-1. Because it effectively shields newborn mice from BPD, IL-1Ra emerges as a promising treatment for a currently irremediable disease that may potentially brighten the prognosis of the tiny preterm patients.