<em>The </em>effect of <em>Salsola tuberculata</em> extracts and Compound A against intracellular interleukin-1alpha (icIL-1α) in the UVB <em>in vitro</em> model for inflammation and chemoprevention in skin — ASN Events
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  2. Poster Session 2
  3. The effect of Salsola tuberculata extracts and Compound A against intracellular interleukin-1alpha (icIL-1α) in the UVB in vitro model for inflammation and chemoprevention in skin

The effect of Salsola tuberculata extracts and Compound A against intracellular interleukin-1alpha (icIL-1α) in the UVB in vitro model for inflammation and chemoprevention in skin (#121)

Tandeka Magcwebeba 1 , Andrea Van Zyl 1 , Amanda Swart 1 , Pieter Swart 1
  1. Biochemistry , Stellenbosch University, Stellenbosch, Western Cape Province, South Africa

Interleukin-1α is a primary cytokine that is constitutively produced by keratinocytes in skin where it facilitates cell proliferation and inflammation (1-3). The chronic production of this cytokine, however, is associated with skin cancer development.  Plant compounds have been shown to prevent chronic inflammation and cancer development in skin by suppressing the pathophysiological production of IL-1α  in vivo (4,5).  An in vitro UVB cell model has also been developed to monitor the anti-inflammatory efficacy of plants and their compounds against IL-α for skin chemoprevention (6).  One of the plants that has been identified for potential use against skin inflammation during chemoprevention is Salsola tuberculata, due the potent anti-inflammatory activity of a synthetic analogue, Compound A (7-8). However, the anti-inflammatory properties of S. tuberculata in skin still need to be elucidated

Aim:  To investigate the effect of S. tuberculata extracts on icIL-1α production in the in vitro UVB skin cell  model of inflammation and chemoprevention and compare activity to Compound A.

Methods: Dichloromethane (DCM) and methanol extracts were prepared from S. tuberculata. The extracts and Compound A were screened for activity (P450 enzyme spectral assay) and cytotoxicity (cell viability assay). Sub-cytotoxic concentrations (IC50) of the extracts and Compound A were used to determine the effect on icIL-1α production, cell viability, proliferation and apoptosis.

Results: The DCM extract had no significant effect in cells. The methanol extract significantly (P<0.05) enhanced UVB induced-icIL-1α production and promoted cytotoxicity. However, the methanol extract had no significant effect on icIL-1α production in non-exposed cells. In contrast, Compound A inhibited UVB induced icIL-1α but also enhanced cytotoxicity.

Conclusion:  Contrary to the anti-inflammatory properties of its synthetic analogue (Compound A), Salsola tuberculata   possesses pro-inflammatory properties that may not be effective against the early stages of inflammation in skin chemoprevention

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