G-CSF and Neutrophils are Non-Redundant Mediators of CNS Autoimmune Disease — ASN Events

G-CSF and Neutrophils are Non-Redundant Mediators of CNS Autoimmune Disease (#255)

Gabrielle L Goldberg 1 , Ann L Cornish 1 , Jane Murphy 1 , Ee Shan Pang 1 , Lyndell L Lim 2 , Ian Campbell 3 , Xiangting Chen 4 , Paul G McMenamin 4 , Brent McKenzie 3 , Ian Wicks 1 5
  1. Inflammation Division, Walter and Eliza Hall Institute, Parkville, Victoria, Australia
  2. Centre for Eye Research Australia, University of Melbourne, Melbourne, Victoria, Australia
  3. CSL Limited, Bio21 Institute, Parkville, Victoria, Australia
  4. Department of Anatomy, Monash University, Clayton, Victoria, Australia
  5. Rheumatology Unit, Royal Melbourne Hospital, Parkville, Victoria, Australia

Granulocyte colony stimulating factor (G-CSF) plays a key role in the regulation of neutrophil production, function and survival. The G-CSF receptor (G-CSF-R) it most highly expressed by neutrophils and myeloid progenitors, and at lower levels by macrophages and monocytes. Here, we investigated the role of G-CSF and neutrophils in two extensively used models of central nervous system (CNS) autoimmunity both of which have long been described as T cell-mediated - experimental autoimmune encephalitis (EAE) and experimental autoimmune uveoretinitis (EAU).In G-CSF-/- mice and in WT mice treated with anti-G-CSF monoclonal antibody (mAb), disease severity in both models was dramatically reduced. A mixed population comprising macrophages ,T cells and neutrophils were identified by flow cytometric analysis of the ocular infiltrate in WT mice with EAU. The eyes of G-CSF-deficient and anti-G-CSF mAb-treated WT mice had reduced disease severity, with markedly reduced neutrophil infiltrate, but little or no change in other myeloid inflammatory cells. In the absence of G-CSF, antigen-specific T cell responses remained intact in both disease models and IL-17A production was maintained.  We demonstrate that G-CSF controls ocular neutrophil infiltrate by modulating the expression of chemokine receptors - CXCR2 and CXCR4 - on peripheral blood neutrophils as well as CXCL2-mediated actin polymerization. These data establish an integral role for G-CSF-driven neutrophil responses in CNS autoimmunity, operating both within and outside the bone marrow and identify G-CSF as a potential therapeutic target in the treatment of human uveoretinitis and multiple sclerosis.