Shedding light on preterm immunity — ASN Events

Shedding light on preterm immunity (#285)

Chun Wang Jason Lao 1 2 , Friederike Beker 3 , Kai König 3 , Elizabeth Noble 3 , Geraldine Walsh 3 , Atul Malhotra 2 4 , Kenneth Tan 2 4 , Gregory Woodhead 3 , Claudia Nold-Petry 1 2 , Marcel F Nold 1 2
  1. Ritchie Centre, MIMR-PHI Institute of Medical Research, Clayton, VIC, Australia
  2. Department of Paediatrics, Monash University, Clayton, VIC, Australia
  3. Department of Paediatrics, Mercy Hospital for Women, Heidelberg, VIC, Australia
  4. Monash Newborn, Monash Health, Melbourne, VIC, Australia

Background: There is little knowledge on the immune system of extremely premature infants. This paucity of evidence impedes advances in the fight against bronchopulmonary dysplasia (BPD), a common, severe chronic lung disease that entails significant morbidity and mortality. No safe and effective treatment exists.

Method:  Blood was collected from extremely preterm infants at 5 timepoints [birth, days 1, 7 and 14 and 36 weeks corrected gestational age (WCGA)]. Following overnight stimulation with PMA/ionomycin, LPS or vehicle in whole blood assays, flow cytometry was used to explore T cells and their polarisation, macrophages and dendritic cells (DC) and their activation status and endothelial progenitor cells (EPC).

Results: In preterm babies, expression of the activation marker MHC II progressively increased over time on macrophages (d1, 48% vs 36-WCGA, 86%) and DC (d1, 24% vs 36-WCGA, 56%). Comparing the one infant that developed BPD with the two that did not at 36-WCGA, we observed a marked increase in macrophage (6-fold) and DC activation (2-fold), but fewer circulating EPC (0.3% vs 1.4% of viable cells). Unexpectedly, we also found that preterm infants are capable of producing the cytokines IFN-gamma and IL-17A.

Conclusions: It only took the results from three preterm infants to reveal that this first-of-its-kind study will revolutionise the understanding of preterm immunity; for example, these data suggest that, contrary to current belief, preterm babies are capable of producing IFN-gamma and IL-17. BPD appears associated with markedly increased cellular activation - a promising finding that may provide a basis for therapeutic innovations.