<em>Indigofera cordifolia</em> seeds attenuate the oxidative stress and inflammation associated with Streptozotocin induced Type 2 Diabetes mellitus — ASN Events

<em>Indigofera cordifolia</em> seeds attenuate the oxidative stress and inflammation associated with Streptozotocin induced Type 2 Diabetes mellitus — ASN Events

Indigofera cordifolia seeds attenuate the oxidative stress and inflammation associated with Streptozotocin induced Type 2 Diabetes mellitus

Indigofera cordifolia seeds attenuate the oxidative stress and inflammation associated with Streptozotocin induced Type 2 Diabetes mellitus (#37)

Padmini Deshpande ¹ , Jayesh Dhodi ¹ , Nitin Gawali ¹ , Pankaj Kothavade ¹ , Amrita Chowdhury ¹ , Archana Juvekar ¹

Department of Pharmaceutical Science and Technology, Institute of Chemical Technology, Mumbai, Maharashtra, India

Aim: The key pathogenic events involved in Type 2 Diabetes mellitus are impaired glucose regulation and insufficient carbohydrate utilization caused either by the lack of sufficient Insulin or resistance to it. The present study was hypothesized to investigate the beneficial effects of Indigofera cordifolia on hyperglycemia-induced oxidative damage in Streptozotocin (STZ) induced diabetic rats.
Methods: Diabetes was induced by an intraperitoneal injection of STZ (45 mg/kg body weight). The seeds of I. Cordifolia were extracted with 70% methanol and fractionated with n-butanol (ICMB fraction). An oral dose of 500 mg/kg of ICMB was given once a day for 14 days following diabetes induction. At the end of the experimental period, blood was obtained from retro-orbital route. Liver and pancreas were used for estimations of antioxidant parameters while biochemical parameters were estimated in serum.
Results: In the diabetic control group, levels of glucose, SGOT, SGPT, Total cholesterol, TNF-α and IL-6 were significantly increased, while levels of SOD and GSH were decreased. Administration of ICMB fraction significantly reversed these alterations. In-vitro antioxidant assays demonstrated the radical scavenging property of ICMB against DPPH and ABTS radicals. Moreover, ICMB caused significant inhibition of the carbohydrate hydrolyzing enzymes α-glucosidase and α-amylase. Histopathological examinations of pancreas demonstrated increase in the number and size of the islets in the ICMB treated group as compared to the diabetic control group.
Conclusion: Thus, it can be safely concluded that Indigofera cordifolia exerts a protective action against STZ induced hyperglycaemia and inflammation.

Authors contributing to this presentation.

Deshpande, P

PADMINI SATISH DESHPANDE 103, Nav-Anjaneya Apt., Gandhari Road, Kalyan (W) CONTACT NO:+91-9004213152 EMAIL ID: deshpande_p12@yahoo.in EDUCATIONAL BACKGROUND • Pursuing Master in Pharmaceutical Sciences (M.Pharm) in Pharmacology from Institute of Chemical Technology, Mumbai under the supervision of Prof. A.R.Juvekar. • Bachelor of Pharmacy (B.Pharm) from Yadavrao Tasgaonkar Institute of Pharmacy, Karjat in April 2012 and secured First Class with Distinction (73.01%). • HSC examination from Birla College of Arts, Science and Commerce, Kalyan (78.50%). • SSC examination at MKES School, Kalyan (85.20%) CO-CURRICULAR ACTIVITIES • Secured an All India Rank 189 in the GPAT 2012 exam conducted by M.S.Baroda, Vadodara, Gujarat. • Secured a percentile of 89 in the MAH-MBA CET exam conducted by the DTE, Maharashtra. IN-PLANT TRAINING: Date: 26th May 2011 to 26th June 2011 Manbro Pharma, M.I.D.C., Dombivali (E) Liquids, External and Capsules Department. Observation of instrumental analysis in the Quality Control Department TECHNICAL ABILITIES: Handling of UV/VIS spectrophotometer, HPTLC, Fluorimeter, ELISA reader, Plethysmometer etc. Dosing in laboratory animals, Herbal drug analysis. Knowledge of Biostatistics softwares INSTAT, PRISM etc. SEMINARS AND PRESENTATIONS: • Poster presentation titled “SGLT2 inhibitors: An emerging target for the treatment of Diabetes Mellitus” at the International Workshop on Research Writing skills at ICT, Mumbai. • Attended a workshop on “ Bioanalytical techniques” at Therapeutic Drug Monitoring laboratory, Mumbai as a part of a seminar organised by SIES college, Sion, Mumbai (September 2013). • Participation in the “7th Young Innovator’s Choice Competition” at ICT, Mumbai (Jan 2013). • Attended seminar on “Increased Drug Failures in market: Safety questionable”, at Bharati Vidyapeeth’s College of Pharmacy, Navi Mumbai (August 2011). • Attended seminar on “A perspective on Green Health supplements” at Y.T.I.P, Karjat (Feb 2011). • Oral presentation for AIDS awareness programme for students at the CHM’s K.M.Kundnani College of Pharmacy, Ulhasnagar (Jan 2011). • Participated in the Free Blood sugar testing and Counselling programme for patients at the Model English High School, Kalyan as a part of Indian Pharmaceutical Association programme (2011). • Poster presentation on “Herbal Remedies” at “3rd Indian Pharmaceutical Association – Student’s Congress” at SVKM’s NMIMS, Mumbai (August 2010). • Poster and paper presentation at the “IVth Young Pharmacist”s Innovative Project Award” at Saraswati Vidya Bhavan”s College of Pharmacy, Dombivali, on “Novel method of Sulphonation using Silica gel.”(Feb 2010). • Attended seminar on “Current Scenario in Pharmaceutical Sciences” at Y.T. Institute of Pharmacy, Karjat (Feb 2009). LIST OF PUBLICATIONS: 1. Publication of research paper titled “Analysis of bioactive components from chloroform and hydroalcoholic extracts of Indigofera cordifolia seeds by GC-MS.” in the World Journal of Pharmacy and Pharmaceutical Sciences in August 2013. 2. Publication of a research paper titled “GC-MS analysis of phytocomponents from petroleum ether extracted oil of Indigofera cordifolia seeds” in the World Journal of Pharmacy and Pharmaceutical Sciences in August 2013. 3. Publication of review paper titled “Synthesis of fused Heterocycles” in the journal “Ultrasonic Sonochemistry” as co-author in June 2013. ADDITIONAL RESEARCH/WORK EXPERIENCE: • Carried out a survey of approximately 300 women in the rural areas of Karjat on a questionnaire pertaining to the Health of the women and Vaccination programme of the children in the villages in the year 2011-2012. • Worked in 2009, on the development of a novel method of Sulphonation of compounds using Silica gel and Sulphuric acid which successfully reduced reaction time and increased the product yield ACHIEVEMENTS AND AWARDS • Awarded the University Grant Commission (UGC) JRF Fellowship for the 2012-2014. • Winner of the second prize (Rs.15000) in the “Solution to Industry Defined Problem” event at Vortex: Chemfest at ICT, Mumbai. • Selected for zonal level in oral and poster presentation on “Current status of women’s and children’s health in rural area near Karjat” at AVISHKAR, Mumbai (Dec 2011). • Winner at Taluka Level Chess Championship and Bronze medal winner at” Kalyan Olympics 2005”and participated in District and State level chess championships. • Ranked first overall in F.Y, S.Y, T.Y and Final Year B.Pharmacy. M.PHARM RESEARCH TOPIC “Evaluation of antidiabetic activity of Indigofera cordifolia plant” under Research superviser Prof. A.R.Juvekar. The project is focussed on the evaluation of antidiabetic activity of Indigofera cordifolia plant. Diabetes is the leading cause of death worldwide, the estimated number of diabetic people by the end of 2020 being 340 million. Although the current therapies involve highly effective synthetic molecules for controlling blood sugar levels, they present toxicity issues. Today, research is focussed on the development of herbal alternatives for better blood sugar control and long term management of DM. Indigofera cordifolia, family Fabaceae, is traditionally used in the treatment of Diabetes and Jaundice. It reportedly possesses antioxidant activity. The present research work focuses on the screening of the plant extracts for hypoglycaemic activity by In-vitro and In-vivo models (In Streptozotocin induced diabetic rats).

Dhodi, J

Gawali, N

Kothavade, P

Chowdhury, A

Juvekar, A