Anti-Arthritic Activity of Petroleum Ether Fraction of <em>Celastrus paniculatus</em> willd. Seeds. — ASN Events
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  3. Anti-Arthritic Activity of Petroleum Ether Fraction of Celastrus paniculatus willd. Seeds.

Anti-Arthritic Activity of Petroleum Ether Fraction of Celastrus paniculatus willd. Seeds. (#100)

Pankaj Kothavade 1 , Vipin Bulani 1 , Dnyaneshwar Nagmoti 1 , Archana Juvekar 1
  1. Department of pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai, M.S., India

Aim: Celastrus paniculatus (CP) is a traditional antirheumatic medicine used in India. Based on previous bioassay guided fractionation study, petroleum ether fraction of CP (PCP) had shown anti-inflammatory activity. Hence, it might be useful in inflammation related disorders like arthritis. The present study was designed to investigate the effect of PCP on adjuvant induced arthritis in rats. 

Method: Adjuvant arthritis was induced in Sprague-Dawley rats by sub-plantar injection of complete freund’s adjuvant into left hind paw. Arthritis was evaluated by arthritic score, paw volume, paw circumference, joint diameter, body weight, histopathological changes and index of thymus and spleen (mg/g). Biochemical analysis in serum were performed which includes superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), nitric oxide (NO), melonaldehyde (MDA), alkaline phosphatase (ALP), aspartate transaminase (AST) and alanine transaminase (ALT). The levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were also determined. 

Result: PCP (100, 200 and 400 mg/kg) significantly ameliorated the arthritis severity in terms of arthritic score, paw volume, paw circumference and joint diameter. Serum levels of NO, MDA, AST, ALP and ALT were significantly decreased when compared with arthritic rats (p < 0.05). The increased levels of IL-6 and TNF-α were significantly reduced in treatment groups.

Conclusion: Our results suggest that, anti-arthritic effect of PCP may be due to immunodepressive effect, cytokine regulation and antioxidant property.